Astrakhan, Russian Federation
The qualitative and quantitative determinations of triterpene saponins content in the fruit of Amorpha fruticosa introduced in the Astrakhan region were carried out. Amorpha fruticosa belongs to the legumes family, used as raw material for leather, paint and soap industry. According to literary data, different parts of the plant Amorpha fruticosa can contain in different ratio essential oil with terpenes, tanning substances, amorphous, apigenin, amorphous eugenol. High-quality definition of saponin in water extraction from fruits of Amorpha fruticosa was carried out on colour reactions of Lafond, reactions with nitrate of sodium and sulfuric acid, and by reactions of foaming and sedimentation. The quantitative content of saponins in shrub amorphous fruits in terms of oleanolic acid was carried out by ultraviolet spectrophotometry in the wavelength range 220-450 nm. The total content of triterpene saponins in the aqueous extraction of shrub fruits Amorpha fruticosa in terms of oleanolic acid was 5,325 ± 0,013 %.
triterpene saponins, introduced plants, water extraction, Amorpha fruticosa, spectrophotometry
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